Biochemical, Molecular Biology, Formulation
This antibiotic is commonly used in molecular biology for selection of positive clones, which carry the kanr gene. The mode of action in on the 70s bacterial ribosome. This can be aliquoted and stored frozen at -20°C. The colorless 200X stock solution is 10μg/ml in H2O, yielding a working concentration of 50μg/ml. Depending on stringency, working concentrations range from 10μg/ml - 50μg/ml.
|Catalog #||Material Name||Company Name||Solute/Solvent|
|1||K1377||Kanamycin sulfate (MW=582.58)||Sigma||Solute|
|2||cat num n/a||MilliQ H2O||Millipore||Solvent|
|3||190-9920||Syringe filter .2mm (25mm)||Nalgene|
10ml stock solution (200x):
Add 100mg of Kanamycin powder in final volume of 10ml H2O and vortex till completely dissolved. Then syringe filter the stock solution.
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