Biochemical, Molecular Biology, Formulation
This antibiotic is commonly used in molecular biology for selection of positive clones, which carry the kanr gene. The mode of action in on the 70s bacterial ribosome. This can be aliquoted and stored frozen at -20°C. The colorless 200X stock solution is 10μg/ml in H2O, yielding a working concentration of 50μg/ml. Depending on stringency, working concentrations range from 10μg/ml - 50μg/ml.
|Catalog #||Material Name||Company Name||Solute/Solvent|
|1||K1377||Kanamycin sulfate (MW=582.58)||Sigma||Solute|
|2||cat num n/a||MilliQ H2O||Millipore||Solvent|
|3||190-9920||Syringe filter .2mm (25mm)||Nalgene|
10ml stock solution (200x):
Add 100mg of Kanamycin powder in final volume of 10ml H2O and vortex till completely dissolved. Then syringe filter the stock solution.
|Eppendorf epMotion 5075 VAC|
|Facilitating true walk-away automation by providing pre-optimized methods for any DNA or RNA purification kit you choose.|
|Antibodies from Epitomics|
|Over 6800 high quality antibodies from Epitomics. All Epitomics antibodies are validated in WB, IHC, ICC/IF, IP and FACS. Learn more|
|MyGenostics, Your Exome|
|GenCapTM technology captures both strands of the Exome with superior evenness for reduced sequencing costs.|