Lipid extraction using the Bligh-Dyer method

WFID: 10263   Date: 08-31-2011  
Protocol Format: Public Draft.
Yuxiang Zheng  
Biochemical, Formulation
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Introduction

The Bligh & Dyer method is an efficient method to extract lipids from biological materials

Materials

Catalog # Material Name Company Name Solute/Solvent
134860MethanolSigmaSolvent
2650498ChloroformSigmaSolvent
3Z2783198 ml reaction vialSigma

Protocol

Place 1 ml of aqueous sample in a 8-ml reaction vial. 
Add 2.5 ml of methanol. 
Add 1.25 ml of CHCl3
Vortex. 
Add 1.25 ml of CHCl3. (note: CHCl3 must be added in two steps)
Add 1.25 ml of water. 
Vortex. 
The bottom organic phase contains the lipids. Transfer this phase to another tube. Evaporate under N2

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