The Bligh & Dyer method is an efficient method to extract lipids from biological materials
|Catalog #||Material Name||Company Name||Solute/Solvent|
|3||Z278319||8 ml reaction vial||Sigma|
Place 1 ml of aqueous sample in a 8-ml reaction vial.
Add 2.5 ml of methanol.
Add 1.25 ml of CHCl3.
Add 1.25 ml of CHCl3. (note: CHCl3 must be added in two steps)
Add 1.25 ml of water.
The bottom organic phase contains the lipids. Transfer this phase to another tube. Evaporate under N2.
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